June 28, 2025

REGULATED INFORMATION Information on the total number of voting rights and shares Mont-Saint-Guibert (Belgium), June 27, 2025, 10:30 pm CET / 4:30 pm ET – In accordance with article  15 of the Law of 2 May 2007 on the disclosure of large shareholdings, Nyxoah SA (Euronext Brussels and Nasdaq: NYXH) publishes the below information following the issue of new shares. Share capital: EUR 6,431,121.38 Total number of securities carrying voting rights: 37,435,640 (all ordinary shares) Total number of voting rights (= denominator): 37,435,640 (all relating to ordinary shares) Number of rights to subscribe to securities carrying voting rights not yet issued: 2,822,816 (all granted subscription rights; this number